How it works

3D-Structured Illumination Microscopy

Podocyte foot processes have a size of only 100-200 nm. Until recently, these cells could only be visualized using the electron microscopy. Now with the aid of the super-resolution microscopy it is possible to make the podocytes foot processes and the filtration slit visible under a fluorescence light microscope.

Kidney sample of a mice with Widefield Microscopy compared to 3D-SIM

PEMP

Kidney slices are stained with an antibody (Nephrin/Podocin) that makes the filtration slit visible. The filtration slit resides between the podocytes.

PEMP calculates a single value for a slice – Filtration Slit Density (FSD). This value describes the ratio between the length of the filtration slit and the surface area.

Simplified a high value describes an intact morphology whereas a low value shows an effacement.

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